FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39794151
Sequences flagged as poor quality0
Sequence length35-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC489050.12289494503853092No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG462890.11632111462812714No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT452070.11360212208070479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG202500.017.23039624
CGTACGG9700.016.5926133
CCTACGT211050.016.58132625
TACGTTC213050.016.44207227
CTACGTT217000.016.22151626
CTCGCTA126550.016.0436067
GACTACC221150.015.94643319
TCGCTAT132350.015.2888058
CCGCGTT43450.015.286977
ACCGCGT43700.015.1211676
CGCTATG134550.015.0388399
ACTACCC236550.014.951797520
TACCCTA239100.014.86449522
CGGCTAT28450.014.64884951
GGTTCTA246700.014.29500966
ACCCTAC256100.013.95833823
TGACTAC258450.013.7508918
CGTGGCT88800.013.53671
CCCGATC174850.013.50625369
CTACCCT264200.013.50379521
Produced by FastQC (version 0.11.5)