FastQCFastQC Report
Thu 12 May 2022
EGAF00005422943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26635050
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT1141160.4284429726995069Illumina Single End PCR Primer 1 (96% over 32bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG578160.2170673604892801No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA413200.15513393066654652No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT384930.1445200966395783No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC313050.11753310018190316No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG303850.11407900492020852No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)