FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19706827
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT511400.25950397798691793TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTC333930.16944889200072644No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT295390.14989221755486057No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA273300.1386829041529618No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG218660.11095647208959616No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA212490.10782557740015682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTA129600.033.267878
ACGCTCA81200.031.4861183
TAGCCGA161950.030.8893079
CTCGCTA80950.027.1850261
ACGTTCG47750.026.5301176
TCGCTAT85600.025.6612852
CGCTATG87900.024.5798473
GTGGGTA212250.024.0910854
GTAGCCG212400.023.8911918
GGGTAGC218350.023.6025926
GGAGTCT204600.023.548723
CAGGCGT192950.022.6060967
TCGGGTG101600.021.9036792
GAGTCTT218400.021.7309384
TGGAGTC223550.021.6172372
GGCGTAG225450.021.5505269
GACGCTC127100.021.5322992
GGTAGCC240400.021.28797
GTCTTGG236900.020.2048916
TGGGTAG254400.020.0878985
Produced by FastQC (version 0.11.5)