FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15749290
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT714260.4535188570405396Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT288020.1828780852978134No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG246100.15626101240119397No Hit
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC198240.12587234091187605No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC194870.12373256191231477No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCC191680.12170707377919894No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCC183870.11674812007398429No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTC181910.1155036195282454No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCC167330.10624605934616736No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC166080.1054523727736298No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA165320.10496981133752696No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG159320.1011601157893467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC75550.035.1082421
GGCGCGT113500.034.8635981
ACGTGCG84350.031.3109538
TAACACG83900.031.0485444
AACACGT99100.026.5767615
AGGTCGA80400.025.7769224
GGTCGAC81300.025.621411
CGCGTGC161500.024.7301143
ACACGTG108900.024.1194786
TGCGCGA91500.024.0670329
GGCGATC30900.024.0256121
GCGTGCC171800.023.121214
CAACCCG198900.022.9391169
CTAACAC117850.022.715333
TCTAACA119400.022.5419182
TGGCGCG98150.021.1158163
CGTGCGT33100.020.4369098
GCGCGTG196650.020.3837852
CGTGCGC136000.020.1608249
CACGTGC142900.019.1365037
Produced by FastQC (version 0.11.5)