FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18990104
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1610920.8482944590508825TruSeq Adapter, Index 13 (97% over 38bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT325230.171262885132172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGA92500.028.8688439
GGAGTCT228750.022.6665763
CTCGCTA77550.021.9394531
TGGAGTC238100.021.595462
GTAGCCG130250.021.054428
GAGTCTT245000.020.9868184
TCGCTAT82150.020.9514472
CGCTATG82200.020.6754383
GTCTTGG256800.020.0404856
AGTCTTG264150.019.9457685
GTGGGTA141400.019.2511624
GGGTAGC147550.018.9273456
GTCGGGT68350.017.7955131
GGTAGCC157550.017.5431567
CGCGTAA6404.7293724E-1116.9687961
TCGGGTG69650.016.6467112
CTTGGAA316550.016.1440358
GTCGGTT23150.015.9499361
TCTTGGA338650.015.5580417
TGGGTAG183850.015.4249215
Produced by FastQC (version 0.11.5)