FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18990104
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT1680330.8848450750980615Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT117500.037.488461
GTCTAAC78450.027.9366871
TAACACG76800.027.7353534
GGCGATC43400.027.5748161
CGCGTGC160300.027.429353
GGTCGAC75050.027.2323197
ACGTGCG79450.026.4490058
GCGCGTG170650.026.1480312
AGGTCGA80850.025.812186
TGCGCGA75400.025.2932179
GCGTGCC182750.024.3351674
TGGCGCG100200.023.3412153
AACACGT94850.023.0642595
GCGACAT66000.022.1344667
GCGATCT62150.020.7283862
TGCGACA69950.020.4725556
GTGCGCG98050.020.0371288
CGTATCA259650.019.8337660-64
GGGCGAT50000.019.7535361
ACACGTG111600.019.6027096
Produced by FastQC (version 0.11.5)