FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005422961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40677845
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT1771930.4356007551530815Illumina Single End PCR Primer 1 (97% over 34bp)
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC554570.13633219753897977No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC492450.12106098540864198No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC439310.10799736318381666No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC424460.10434672731556945No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC410910.10101567573208463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)