FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19899344
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT1062360.5338668450578069Illumina Single End PCR Primer 1 (96% over 32bp)
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC289230.14534649986451814No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC260220.13076812984387828No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC252890.1270845913312519No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG244070.12265228441701395No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC239480.12034567571674724No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC234030.11760689196588592No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG200400.10070683737112138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT136900.036.8590351
AGGTCGA121450.029.507764
GGTCGAC132050.028.2774831
CGCGTGC194550.026.0405543
GGCGATC43250.025.83421
GCGCGTG200000.025.6207332
GGGCGAT38150.025.5087181
TGGCGCG117600.023.8652533
GCGTGCC218250.023.3114664
GTCTAAC70400.022.629191
TAACACG67850.021.9545694
ACGTGCG71850.021.0341668
GTCGACC185150.020.4846442
ACCCGTG196650.020.3466996
TCGACCC188250.020.1457943
CGACCCG200500.019.919714
CGTGCCT257950.019.7515455
GCGACAT61250.019.621757
TGCGCGA67550.019.2837989
GCGATCT63000.018.5061472
Produced by FastQC (version 0.11.5)