FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16470740
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT642920.3903406890036513TruSeq Adapter, Index 7 (97% over 36bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT267620.16248207427231565No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA190830.11586000386139299No Hit
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG189200.11487037012301816No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG181780.11036541163299281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA58400.027.1423971
TAGCCGA93450.025.9572539
TCGCTAT60800.025.8196812
CGCTATG60850.025.5617073
GGAGTCT194500.022.2140793
GTCGGGT56500.022.1622871
TGGAGTC205300.021.1507782
GTAGCCG120650.020.4632648
GTCTTGG213900.020.190576
GAGTCTT215200.020.0772574
AGTCTTG225450.019.315535
GTGGGTA129750.019.147284
TCGGGTG64150.018.6341442
GGGTAGC142400.017.9442986
GCGGGTT24950.017.6960451
CGGGTAT20300.017.1144181
GTCGGTT22450.016.7650131
GGTAGCC152150.016.6523747
CTTGGAA256200.016.29518
TCGACCC20950.015.4677272
Produced by FastQC (version 0.11.5)