FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005422975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005422975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16470740
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT661170.4014209440498727Illumina Single End PCR Primer 1 (96% over 32bp)
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC300960.18272403061428932No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC300770.18260867453435609No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC276640.16795845238283164No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC271780.1650077652855913No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC256610.15579749300881443No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG237290.14406760109138994No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG227500.13812372728851283No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC213140.12940523619460936No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGA128450.037.2574126
GGTCGAC145150.032.7731977
GGCGCGT93900.031.5526961
GGGCGAT31150.029.1590081
GGCGATC37250.029.0285431
GTCGACC189000.025.2839348
TCGACCC191000.024.9439749
CGCGTGC131200.022.2153
GCGACAT58900.021.6268047
TGCGACA59250.021.2556726
CAGGTCG233200.020.7373245
CGACCCG216650.020.72773
GCGTGCC140800.020.699844
ACCCGTG217150.020.6793425
CGCCGTA98450.020.36646355-59
TGCAGGT237500.020.150383
GCGCGTG150700.019.8669092
CGTATCA103400.019.4088760-64
GCCGTAT103250.019.39176455-59
CGACCTA17100.018.9378644
Produced by FastQC (version 0.11.5)