FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005423376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005423376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642900043
Sequences flagged as poor quality0
Sequence length35-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCCG202788483.154276970549215TruSeq Adapter, Index 8 (96% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCCCGTATGCCG6840530.10640114391779562TruSeq Adapter, Index 8 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)