FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005423380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005423380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543380135
Sequences flagged as poor quality0
Sequence length35-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCCG223550694.1140754989138495TruSeq Adapter, Index 7 (96% over 32bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN184213233.390135526393507No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC6217530.11442321129387625TruSeq Adapter, Index 7 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)