FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61699877
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG88950.015.50549948
CGTATCG39650.014.607063
TCGTATG97600.014.20792645
CTCGTAT100850.013.78406344
CGTATGC104800.013.16134646
TATCTCG110550.012.88630841
GATCGGA114800.012.2243361
TCTCGTA115950.012.196774543
ACACGTC126500.011.690351513
GTATCGA53100.011.6205324
CCGGGAT121250.011.033741
ATCTCGT129000.010.96261342
CGCAAAT60600.010.6400021
CGACAAT80400.010.4213271
CGCGGAT37350.010.3395151
CGATAAC63100.010.218451
CTCGGTT106400.010.2080241
ACGTCTG146400.010.077764515
TATATCG50400.010.06034235
CTCGGAT152850.010.0384651
Produced by FastQC (version 0.11.5)