FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61699877
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG39000.015.1168173
CGTATCA67200.014.99260150
GCCGTAT81700.012.50091848
GTATCGA52850.012.3299784
CGCCGTA86550.011.91930547
CGCGGAT40800.010.9005081
CTCGTTT118650.010.6057731
CGACCGT31650.010.56611251
CCGGGAT118750.010.5097451
TCGCCGT101800.010.40317546
CGCGAAT35850.010.0975811
CCCGTAT82350.010.0895231
CGACGAT50350.010.0654941
GATCGGA112600.09.9505322
CGCAAAT64450.09.7891591
CCGTATC106750.09.76127849
CGCGTAT12600.09.57667351
CGATAAC62400.09.5582581
CTCGGGT125850.09.5332991
CGCAATT58600.09.5309151
Produced by FastQC (version 0.11.5)