FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87877995
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA116150.028.75015850
GCCGTAT131250.025.96908248
CGCCGTA139800.024.30801647
GCGTCGT145250.023.87790112
GATCGGA175700.021.3062922
CCGTATC175250.019.60629749
TCGCCGT177850.019.22384546
AGCGTCG185300.018.773211
TGGTCGC185000.018.40681643
TCGTGTA204800.017.14872615
AGATCGG205250.017.0785311
ATCGGAA213000.017.0264513
AGAGCGT212200.016.586879
CGTGTAG217550.016.49258616
GTCGTGT217000.016.3757314
CGTCGTG218600.016.29025313
GTAGATC228900.015.61233632
GTCGCCG225400.015.52091545
GAGCGTC239650.014.6731310
GGTCGCC243750.014.12644
Produced by FastQC (version 0.11.5)