FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127951800
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG304200.034.0936846
CGTATGC321450.032.12328347
CTCGTAT323550.031.98523745
TATGCCG327100.031.60046249
CGTATCT335450.030.73547440
AGATCGG358200.028.0423181
TCTCGTA368100.027.96147544
GTTCCGT378350.027.8082636
TATCTCG376800.027.39043842
TTCCGTA386300.027.32629637
ACACGTC408500.026.72220614
GATCGGA382150.026.4083372
ATCTCGT400800.025.99537843
AGTTCCG427600.025.36403835
ACGTCTG436150.024.98709516
TCCGTAT449950.023.2203738
ATCGGAA439150.022.9330653
CACGTCT481450.022.8026715
CCGTATC457000.022.7834239
CACACGT497100.022.06437713
Produced by FastQC (version 0.11.5)