FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127951800
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT245100.040.96355448
CGTATCA253600.039.7767650
GCGTCGT276600.037.713312
CGCCGTA308700.032.65811547
GATCGGA327150.032.272342
TGGTCGC312450.031.90451643
TCGCCGT313300.031.648446
AGCGTCG346450.030.4119711
AGAGCGT362150.029.0519569
CGTCGTG364700.028.81496813
GTCGCCG359850.028.24587845
AGATCGG371950.027.813631
ATCGGAA389450.027.2437323
CCGTATC387000.026.38361749
GTAGATC394500.026.37414232
TCGTGTA413550.025.428315
GTCGTGT414800.025.29259714
TCTCGGT420700.024.48136137
CGTGTAG435150.024.3348516
GAGCGTC446900.023.57756810
Produced by FastQC (version 0.11.5)