FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163549044
Sequences flagged as poor quality0
Sequence length50-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT38488782.3533479046199743TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTA30534871.8670161104702026TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG3923550.057.965211
CTCGTAT4946550.054.55130844
ATCGGAA8600700.034.6984522
TCGGAAG8708650.034.1654933
CGGAAGA8757900.033.9268844
CGCGTAT39700.033.3859744
GATCGGA8514000.033.195351
ACACGTC8607050.032.35660613
AGAGCAC9225000.032.1420828
CGTCTGA8611850.032.13558216
ACGTCTG8636850.032.07929615
GCACACG8754000.032.03983711
CACGTCT8688550.031.95448114
CACACGT8769450.031.9532612
TCTCGTA8462050.031.84451743
CACTCCG8408150.031.7293531
AATCTCG8515600.031.72255741
ATCTCGT8551800.031.52884142
ACTCCGG8460450.031.51496332
CCGGAGA8657850.031.44816635
Produced by FastQC (version 0.11.5)