EGAF00005431779 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005431779
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	163549044
Sequences flagged as poor quality	0
Sequence length	50-75
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGAAA	2453344	1.5000662431264349	Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATTT	330641	0.20216626885327438	Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGGCTATAGTGTAGAAA	214035	0.13086900098297122	Illumina Single End PCR Primer 1 (97% over 35bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	184523	0.11282426083762372	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGGCTATAGTGTAGATTT	174139	0.10647509501798127	Illumina Single End PCR Primer 1 (97% over 34bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	393070	57.66883	1
CGGCGTG	133490	55.588604	12
GCGGCGT	91615	54.83877	11
ACGGCGT	48175	52.691307	11
AGCGGCG	98420	51.375008	10
GCGTGTA	57665	48.067554	14
GGCGTGT	92205	47.86618	13
AGACGGC	100985	47.766876	9
GTAGAAA	426580	47.568462	44
GATCGGG	59845	47.54411	1
TGTAGAA	416880	47.406914	43
GAGTGTA	406620	45.762955	28
ATCGGGA	62120	45.660023	2
GCATACG	42060	45.596684	14
TGTAGGC	408205	45.482647	31
GACGGCG	43185	45.391422	10
CGGCATA	44155	44.95497	12
GCGTGCG	33985	44.701843	14
AAGACGG	109870	44.62083	8
CTATAGT	481240	44.49421	37