FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161763130
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG190650.024.69516244
CTCGTAT210150.022.32015643
TATGCCG212650.022.23336647
CGTATGC216100.021.96576945
TATATCG287850.019.36810935
GCGCTAA295850.017.89582342
ATATCGC316600.017.80757536
CCGTCAT324500.017.6926651
TCTCGTA268150.017.50553942
CGCTAAG322250.016.5821943
AATCTCG294400.016.39789640
ACACGTC304750.016.18453214
AGCGCTA345250.015.42680541
AGATCGG292950.015.3219511
ATCTCGT328050.014.74800441
ACGTCTG349900.014.42885116
TACTCTA394600.014.2758599
GACGCTA158400.014.2264364
GTATATC433450.013.2964834
GATCGGA347150.013.1092692
Produced by FastQC (version 0.11.5)