FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161763130
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT189650.023.82119648
CGTATCA202800.022.5665650
GCGTCGT214850.021.94194212
TATATCG290400.019.67804335
CCGTATC243300.019.15644349
CCGTCAT337200.018.6616941
GCGCTAA311600.018.3509942
ATATCGC322150.017.81585936
CGCTAAG338250.017.07949343
TCGCCGT263100.016.96626346
AGCGTCG280100.016.8551411
TGGTCGC273950.016.7683943
CGCCGTA278800.016.43449647
CGTCGTG298900.016.15870513
AGCGCTA359550.015.97242341
AGATCGG304800.015.3605571
TACTCTA396050.015.3569229
GATCGGA313450.015.2709032
AGAGCGT321600.014.8641179
GTCGCCG314800.014.83653445
Produced by FastQC (version 0.11.5)