FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88903998
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA16455651.850946005825295TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5552100.6245050981846734TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2440700.050.2076947
TCGTATG2461350.049.8653444
CGGCTTA2465600.049.7443532
CGTATGC2488050.049.34150745
CTCGTAT2484800.049.14775543
GTATGCC2551350.048.06578446
GCTTAAG2572250.047.7586134
GGCTTAA2578450.047.63678733
AGTCACG2573350.047.63239327
ACACGTC2588200.047.58411412
ACGGCTT2585450.047.44077731
TCTCGTA2486750.047.4181442
ACGTCTG2594900.047.4133414
CGTCTGA2593950.047.38681815
TAAGATC2489700.047.34636737
ATGCCGT2587450.047.32069448
TCACGGC2621600.046.80949829
GCCGTCT2611450.046.7021750
ATCTCGT2531150.046.58226841
GTCACGG2641700.046.42721628
Produced by FastQC (version 0.11.5)