FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96467477
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA101150.015.39305850
GCCGTAT115450.013.367208548
CGCCGTA117700.012.72802947
GCGTCGT138250.011.35887312
TATATCG66300.010.82572535
CGTATCG69700.010.80170253
GATCGGA147550.010.7276632
CTCGGAT236150.010.0844481
CCGTATC158650.010.0349849
CTCGAAT160900.09.8816841
CGACGAT72800.09.8136361
CTCGGTT163600.09.7614141
CTCGTAT81600.09.7424191
TCGCCGT156150.09.73003446
GACGCTA87600.09.7141484
CCCGTAT99150.09.7133971
TCGTGTA174400.09.64659415
GTCGTGT177250.09.5506614
CGTCGTG178200.09.49973613
TCCGATC105100.09.4967131
Produced by FastQC (version 0.11.5)