FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104296977
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1358560.13025880893940003TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG443700.035.3736531
TCGTATG478450.032.50316644
CTCGTAT476150.032.13208843
TATGCCG493850.031.41081847
CGTATGC499850.031.14331445
GTCACGA519500.030.07783130
TCTCGTA520650.029.22637242
AGTCACG551550.028.33476629
ATCTCGT547450.027.87094141
ACACGTC568200.027.74134814
TCACGAT577650.027.17750231
ACGATCA579000.027.09498833
GATCGGA581900.027.0565662
ACGTCTG586850.026.84185216
CACGATC613050.025.67903332
CGATCAG611550.025.5877634
ATCGGAA618150.025.4471453
CACGTCT634000.024.96001615
CACACGT640550.024.74261713
TCGGAAG658800.023.9396844
Produced by FastQC (version 0.11.5)