FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104296977
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA364100.041.76289450
GCCGTAT383300.039.8493548
CGCCGTA416900.036.57080547
TCGCCGT418600.036.11544846
GCGTCGT435250.035.50180412
AGATCGG440100.034.9615631
CCGTATC443650.034.4626149
TGGTCGC453900.033.25359343
AGCGTCG472800.032.71841411
CGTCGTG480900.032.37628613
GTCGCCG484700.031.63804445
GTATCAT496650.031.00834751
AGAGCGT505200.030.6066239
GTAGATC509850.030.44115632
GATCGGA507900.030.439452
TCGTGTA536150.028.86111315
GTCGTGT539850.028.72504814
TCGGTGG541950.028.57084839
TCTCGGT541250.028.51559337
GAGCGTC543450.028.46030410
Produced by FastQC (version 0.11.5)