FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155796512
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2403160.15424992313049987TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG611900.033.26033445
TATGCCG613800.033.1639948
CTCGTAT609500.033.1140544
CGTATGC638350.031.97388546
TCGAACG365750.031.7572940
TATCTCG672350.029.55185741
GATCGGA719900.029.140671
ACACGTC721600.028.8937113
ACGGTAA407250.028.5615944
ATCTCGT712350.027.73770542
TTGAACG444000.027.6715537
CGGTAAC424750.027.40914345
AACGGTA425750.027.28810143
ACGTCTG768200.027.21698415
GTCGAAC433250.026.93863139
TCTCGTA754700.026.16261743
CGAACGG452200.025.69970741
CACGTCT818600.025.53335414
ATGCCGT796050.025.52304849
AGTCGAA468350.025.10437438
Produced by FastQC (version 0.11.5)