FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155796512
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA575300.035.1995949
GCCGTAT589850.034.4960747
TCGCCGT637250.031.93070245
TCGAACG340600.031.1654140
CGCCGTA667250.030.39346546
GTCGCCG674700.030.29112244
CCGTATC683950.029.6486848
TGGTCGC692200.029.4892142
GCGTCGT708000.029.03813211
GATCGGA698350.028.5298521
ACGGTAA385600.027.70864144
AGAGCGT756800.027.1514728
CGTCGTG758450.027.05235712
CGGTAAC402650.026.49264545
TTGAACG428450.026.4311437
GTCGAAC404350.026.37989839
AACGGTA408000.026.1526643
TCGTGTA777550.025.92639714
GTATCAT797500.025.69537450
GTAGATC811800.025.56539531
Produced by FastQC (version 0.11.5)