FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50572997
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC57600.024.25182244
TCGTATG61450.022.80377843
TCGAACG60900.022.1049940
CTCGTAT65200.020.88454442
ACGGTAA70300.019.49511544
TATCTCG71150.019.01332739
GATCGGA75850.018.5226841
AACGGTA75700.018.01710743
TTGAACG82700.017.7132367
GTCGAAC76600.017.68926839
CGAACGG78450.017.38422841
AGTCGAA80750.016.97107938
GAACGGT81250.016.7319742
GTCACGC84800.016.65159629
CGCCAAT86800.016.4219233
AGTCACG86200.016.35591128
GAACGCT89150.015.7650359
ATCTCGT87300.015.69748140
ACACGTC90650.015.64982413
TCGACTA31400.015.41296444
Produced by FastQC (version 0.11.5)