FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143255805
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2317140.1617484192001853TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2056660.1435655609208995TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG525300.036.3187181
TCGAACG362300.029.59392440
CTCGTAT658050.028.61122345
TATGCCG667250.028.47521449
TTGAACG406600.027.8926837
TCGTATG684400.027.80685846
TATCTCG673500.026.95109642
ACGGTAA398550.026.8829944
CGTATGC720650.026.52867947
GTCACGA726650.026.16821730
CGGTAAC410200.026.14489445
GATCGGA743200.025.7102972
CACGAGT745750.025.55373832
ACACGTC750750.025.52568814
AGTCACG767850.024.78662929
ATCTCGT736200.024.758643
AACGGTA439300.024.42856243
GTCGAAC443800.024.2615939
TCACGAG792100.024.07969531
ACGTCTG801900.023.93572616
Produced by FastQC (version 0.11.5)