FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143255805
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG527000.035.6719361
CGTATCA622150.030.00432850
GCCGTAT654950.028.470148
TCGAACG334150.028.01938440
TCGCCGT690650.027.17559246
CGCCGTA706600.026.47736247
CCGTATC713750.026.22110649
TTGAACG383450.025.8721837
GTCGCCG732100.025.81428145
GATCGGA737400.025.5419162
ACGGTAA371100.025.360744
TGGTCGC754650.025.11067443
CGGTAAC383000.024.54574445
TCGTGTA772150.024.28905515
GTCGTGT792600.023.71917714
AGAGCGT797600.023.660889
GCGTCGT792300.023.50209812
AACGGTA409750.023.01833343
GTCGAAC413700.022.78990639
GGTCGCC837200.022.60243644
Produced by FastQC (version 0.11.5)