FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127305301
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG186650.029.36865240
ACGGTAA219900.025.03616544
TCGTATG270650.024.16522243
AACGGTA227450.024.11413243
CGAACGG229000.024.10146341
CGGTAAC244150.022.77561845
CTCGTAT286400.022.58353842
GTCGAAC250000.022.35556439
CGTATGC295550.022.2576844
TATGCCG298850.021.85027946
TTGAACG287100.020.9767537
GAACGGT272350.020.48077442
GCCTAAC281350.020.02227424
GAACGCT291000.019.8055159
AGTCGAA291850.019.51662338
GATCGGA354250.018.6212331
GGCCTAA327800.017.35268623
ACGATCT367150.017.2242137
ACACGTC402550.016.81916813
AACGCTG346000.016.73693310
Produced by FastQC (version 0.11.5)