FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127305301
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG185150.029.4201240
ACGGTAA218850.024.7964744
CGTATCA263750.024.43319949
AACGGTA224250.024.29078343
CGAACGG233250.023.44195641
CGGTAAC236150.023.06769845
GTCGAAC241750.022.81741539
GCCGTAT282450.022.571947
TTGAACG276300.021.2110677
GAACGGT270550.020.36320542
CGCCGTA319400.020.05912646
GAACGCT285200.019.9202429
GCCTAAC281700.019.9110824
AGTCGAA290750.019.11392638
CCGTATC346850.018.53978748
GGCCTAA324700.017.51837523
GATCGGA374700.017.303071
GCGTCGT378350.016.95816811
AACGCTG346050.016.68621810
TCGTGTA382450.016.67737214
Produced by FastQC (version 0.11.5)