FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126125722
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT388400.022.73678842-43
TATGCCG415750.021.332246-47
CGTATGC423200.021.0553944-45
ATCTCGT502250.017.81963340-41
CGTATCG148050.015.5259743
TCGTATG392050.015.4012242-43
CACGTCT642100.014.33144214-15
GTATCGA169250.013.9415274
AATCTCG444800.013.54429738-39
CACACGT700200.013.20591412-13
TCTCGTA458800.013.18524540-41
GATCGGA901700.013.17824651
CCGTCTT698350.013.03696450-51
CGTCTGA712550.012.99009616-17
ATCGGAA927250.012.7446222
CCCGTAT220250.012.671891
CGCGGGT236500.011.9204041
CGACCGT97800.011.9147171
TCGGAAG1012000.011.8110933
ACCAATC791450.011.716228536-37
Produced by FastQC (version 0.11.5)