FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117309225
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA376900.044.46946750
GCCGTAT396750.042.52619648
CGCCGTA408950.041.2314647
GCGTCGT456250.037.66720612
TGGTCGC445450.037.4542243
TCGCCGT447950.037.36218346
CCGTATC464850.036.44949
AGCGTCG498450.034.63002411
CGTCGTG510550.033.7766113
GATCGGA521150.033.2266732
AGATCGG523400.032.905851
GTAGATC535500.032.0113632
TCGTGTA537150.031.84369515
AGAGCGT546450.031.7281849
GTCGCCG535450.031.67350645
ATCGGAA562150.030.7467823
GTATCAT552750.030.73683751
GTCGTGT559700.030.73070114
CGTGTAG566100.030.34057416
TCTCGGT582150.029.30266437
Produced by FastQC (version 0.11.5)