FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005431820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56882473
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCTATGTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA20525593.608420822350674Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCTATGTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA4258510.7486506432306486Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)