FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62562797
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA21903743.5010806821824154TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3594500.5745427270459151TruSeq Adapter, Index 8 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2768850.058.18968244
TATGCCG2770400.058.11129847
CGTATGC2802600.057.47386645
CTCGTAT2782700.057.4196443
AATCTCG2763400.056.96190340
GTATGCC2834850.056.82422346
AGTCACG2838800.056.7431827
ACGCTTG2845700.056.6847931
GTCACGC2850600.056.49130228
TCTCGTA2788500.056.48232742
ATGCCGT2850650.056.39235348
CGTCTGA2880300.056.1587715
GCCGTCT2858250.056.1092750
ATCTCGT2810250.056.06279441
CACGCTT2884250.055.91356730
ACGTCTG2907350.055.68048514
ACACGTC2916550.055.5865512
TCACGCT2910400.055.42066629
TGCCGTC2909250.055.22295449
CGCTTGT2927200.055.20900332
Produced by FastQC (version 0.11.5)