FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62562797
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATGTTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA22378683.576994807313362Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATGTTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA3650220.5834489784719823Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2762150.058.2605658
CGCCGTA2761300.058.18928557
CGTATCA2776300.058.04173360
CCGTATC2791600.057.62123559
TCTCGGT2810000.057.39461547
GTAGATC2828150.057.1547342
TAGATCT2831200.057.090743
GTATCAT2842950.056.70391561
TCGCCGT2849100.056.36246556
ATCATTA2866500.056.3079663
GTCGCCG2853950.056.2227455
GTAGGGA2877200.056.20576517
GTATGTT2874950.056.1879532
GTGTAGA2886100.056.09900740
TGTAGGG2888600.056.00662616
TATGTTC2886600.055.9481933
GCGTCGT2892750.055.94290510
GTGTAGG2896200.055.86992615
TTCGTGT2891000.055.8451537
CGGTGGT2881200.055.8441850