FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121123915
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG158800.019.2315653
GTATCGA176650.017.8724524
CTCGTAT301650.017.04218542-43
TATGCCG315900.016.31863446-47
CCCGTAT230450.015.6599271
CGTATGC333800.015.56924844-45
CCGTATC254800.013.71717552
ATCTCGT433800.012.23329840-41
CGACCGT94800.011.4996571
TATCGAA285350.011.4259645
CGCGGGT229300.011.2710361
ATCGAAG392300.010.8423276
CACGTCT530650.010.03254914-15
CCGGGAT329950.09.9690811
CACACGT565250.09.53033912-13
CGCGGAT118900.09.4058991
TCGTATG314450.09.31430342-43
CTCGGGT294550.09.3006811
GACGCTA145500.09.1244384
CTCGGAT379100.09.1228231
Produced by FastQC (version 0.11.5)