FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142170280
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6711130.47204872917180724TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3195600.2247727162104485TruSeq Adapter, Index 2 (100% over 63bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG1781580.12531311044755628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG1071650.051.8663671
TCGTATG1357450.040.53180344
CTCGTAT1353250.040.36447543
CGTATGC1381900.039.84211745
TATGCCG1379850.039.76468347
TATCTCG1337550.039.2753940
ACACGTC1464000.037.9145714
GATCGGA1471150.037.8947142
ACCGATG1455400.037.76425633
ATCTCGT1404100.037.4827241
CGATGTA1488750.036.84453235
ACGTCTG1516800.036.58484316
CACCGAT1514400.036.28626332
TTGAACG595200.036.1207287
TCACCGA1550800.035.6027831
CCGATGT1552100.035.3904334
ATGCCGT1543550.035.38220648
CACGTCT1583200.035.0897215
GTATGCC1580900.034.86375846
GTCACCG1591350.034.70034430
Produced by FastQC (version 0.11.5)