FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142170280
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAA2718860.19123968806982725Illumina Single End PCR Primer 1 (100% over 58bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG1533050.1078319603787796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG1061800.052.0432661
CGTATCA1332550.040.7288650
GCCGTAT1343500.040.37655648
GATCGGA1437200.038.561532
TCGCCGT1426900.038.05375346
CCGTATC1442100.037.68952649
AGAGCGT1462850.037.551249
TGGTCGC1477200.037.11173643
GTCGCCG1473200.037.1085745
GCGTCGT1478500.036.97057712
GTATCAT1481500.036.79659351
GTAGATC1531450.036.1770432
CGCCGTA1517500.035.767747
TCGTGTA1552800.035.2655715
GTCGTGT1567250.034.95628714
CGTCGTG1572500.034.8423113
AAGAGCG1598750.034.7567188
GAGTGTA1600500.034.4978428
TCGGAAG1611750.034.496834
TCGGTGG1609950.034.33175739
Produced by FastQC (version 0.11.5)