FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107119288
Sequences flagged as poor quality0
Sequence length50-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT122350.028.74390644
TCGTATC145100.023.8178438
GATCGGA157800.022.6873261
TATCTCG153350.022.51612341
ATTCGTA161550.021.5056836
GTCACGA163850.021.4962429
CGTATCT172350.020.01647239
TCTCGTA176300.019.84455143
ACGAATT179250.019.60276832
ATCGGAA186900.019.2850842
AATTCGT181150.019.2461935
GAATTCG185100.019.04970634
CACGAAT184650.019.04590631
CGAATTC186150.018.89284733
CGTCTGA194550.018.4310716
ATCTCGT194300.018.25735742
TTCGTAT193650.018.19321437
ACACGTC201200.017.86024713
AGTCACG201050.017.80687528
AGATCGG119700.017.7662261
Produced by FastQC (version 0.11.5)