FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107119288
Sequences flagged as poor quality0
Sequence length50-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT70550.026.99582712
CGTCGTG82950.023.0339513
AGCGTCG85350.022.84960211
TCGTGTA100350.018.61741415
GTCGTGT107000.017.82884414
GATCGGA116400.017.1898212
AGATCGG119400.017.1042671
ATCGGAA139950.014.3635363
CGTGTAG142700.013.34891516
AGAGCGT150700.013.2732139
GAGCCGT150150.012.419937
GAGCGTC165900.011.98150610
CCGTGTA116050.011.44026740
AAGAGCG190800.010.441268
AGAGCCG220050.09.126760536
TCGGAAG234250.08.9074614
AGCCGTG204750.08.86942938
GCCGTGT170100.08.48712639
CGGAAGA260250.08.1353165
GTAGGGA321100.06.70799519
Produced by FastQC (version 0.11.5)