FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66618360
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7272861.0917200603557338TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4143480.621972681404946TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA957850.054.919191
TCGTATG1245150.041.95425845
TATGCCG1244500.041.95207248
CTCGTAT1264850.041.27323544
CGTATGC1265150.041.2725946
TATCTCG1255050.041.09361641
GTATGCC1316100.039.70379347
ACACGTC1327150.039.5222413
ACGTCTG1327200.039.4579815
TCTCGTA1311850.039.3847243
CGTCTGA1338800.039.13671516
ATCTCGT1334800.038.6894642
GCCGTCT1352200.038.57472251
ATGCCGT1359600.038.3776849
CACACGT1371000.038.29379312
CACGTCT1374800.038.16998314
ATATCTC1367650.037.77323540
ATCGGAA1409800.037.3407022
CCGTCTT1400400.037.24907352
TGCCGTC1403450.037.23021750
Produced by FastQC (version 0.11.5)