FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005431833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66618360
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA7478851.1226409656437053Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA4283810.6430374449325982Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA973950.054.0894971
CGCCGTA1236150.042.2268358
GCCGTAT1245500.041.91597459
CGTATCA1251550.041.6992261
CGTGTAG1273800.041.10719315
CCGTATC1271900.041.06493860
GCGTCGT1281600.040.95461711
GTAGATC1287550.040.6305343
TCTCGGT1290250.040.51381748
TCGTGTA1296500.040.44340514
TCGCCGT1291950.040.42209257
GTCGTGT1310700.040.0482613
TGGTCGC1310500.039.93924754
GGTCGCC1311550.039.87044555
GTATCAT1312900.039.81389262
AGCGTCG1321750.039.77581410
GTAGGGA1318950.039.72109618
CGTCGTG1323100.039.66507712
TAGATCT1318950.039.66324644
GTCGCCG1319050.039.6280556
Produced by FastQC (version 0.11.5)