FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79177413
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA132400.019.4509814
CGTATCG128500.019.2017043
CCCGTAT170800.016.2026391
CCGTATC184250.014.5635452
TATCGAA221350.012.3763215
ATCGAAG275500.011.5958086
CGACCGT70650.011.2391351
CGCGGGT143000.011.039811
GACGCTA96850.010.897834
ACGCTAC107850.010.0908115
CCGGGAT205400.09.9276761
CGGGGAT232300.09.6882341
TCGAAGG334050.09.6194767
CTCGGAT242450.09.37953951
CCGCAAT97500.09.0596531
CGCGGAT80100.09.0333021
CTCGGTT153250.08.9524251
CGGGGTT266400.08.9419461
CGCCAGT235700.08.7511131
GATCGGA451450.08.5814294
Produced by FastQC (version 0.11.5)