FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145156391
Sequences flagged as poor quality0
Sequence length50-75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA182750.016.8292271
GCGTCGT118600.016.25403411
AGCGTCG143550.015.83551110
GTACGTC109650.014.91134432
CGTCGTG134400.014.45929312
ATCGGAA221900.013.9399862
ACTACCG102300.013.79948521
TACGTCC129350.012.71340933
GTGTACG135950.012.66433630
CGTGTAG192950.012.39950815
AGATCGG164900.012.3069591
TCGTGTA154700.012.19771415
GTCGTGT178600.011.33797413
TACCGCA125650.011.08568823
CCGCATT131700.010.591325
GGGTACG100450.010.37218629
CTACCGC139100.010.21666222
AGAGCGT234150.010.0565588
TGTACGT160350.09.921731
GATCGGG140500.09.838191
Produced by FastQC (version 0.11.5)