FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142573613
Sequences flagged as poor quality0
Sequence length50-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT214950.037.25612344
GATCGGA261400.031.573161
TATCTCG264850.030.30479241
TCTCGTA282800.028.47453143
ATCGGAA310900.026.6376082
CGTCTGA311550.026.37284316
ACACGTC313200.026.14697313
ATCTCGT318700.025.24848742
ACGTCTG335900.024.5314615
TCGGAAG377300.022.0623913
CACGTCT391000.021.22844914
GCACACG401200.020.67472611
CACACGT415600.020.04602812
CGGAAGA422950.019.7372654
AGCTATC497350.016.62600938
GCTATCT531200.015.6608339
AGATCGG142850.014.1376531
GAAGCTA641750.013.22102536
AAGCTAT650750.013.12461837
CTATCTC667050.012.66596740
Produced by FastQC (version 0.11.5)