FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142422773
Sequences flagged as poor quality0
Sequence length50-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT96650.018.7553512
CGTCGTG106400.017.02921713
AGCGTCG113450.016.05598611
AGATCGG146500.012.9790491
GATCGGA161750.012.9455531
GTCGTGT146150.012.29604214
TCGTGTA145900.012.23635615
ATCGGAA198300.010.8221412
GAGCCGT191900.09.60668737
CCGTGTA158650.09.46362140
GAGCGTC197100.09.44464510
CGTGTAG199900.09.29690616
AGAGCGT205400.09.0917639
GATCGGG99000.08.0774451
AAGAGCG237900.07.9516618
GCCGTGT217700.07.509519639
TCGGAAG298050.07.4590633
AGCCGTG258350.07.290387638
AGAGCCG282600.07.06577136
CGGAAGA347350.06.7245354
Produced by FastQC (version 0.11.5)