FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160056105
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4156030.2596608233094264TruSeq Adapter, Index 6 (100% over 63bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG2382390.1488471808057556No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2175800.13593983184833844TruSeq Adapter, Index 6 (100% over 63bp)
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGGGGT1972090.12321241979492129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT936200.040.7777942
TCGTATG974700.039.6643
TATGCCG981450.039.0291746
GATCGGA1022150.038.4731181
TATCTCG957750.038.44885339
CGTATGC1014700.038.07497444
CGCCAAT1013450.038.00706533
TTGAACG761400.037.561757
ACACGTC1043200.037.47514713
AGTCACG1048100.036.897828
ATCTCGT1011900.036.52530740
GTCACGC1064250.036.25446329
ACGTCTG1101350.035.46907815
AACGCTG815350.034.6746410
ATGCCGT1104850.034.59445647
CACGTCT1143800.034.2635514
GTATGCC1159550.033.2278245
AGATCGG538000.032.9995771
ACGCCAA1185500.032.77685532
GCCGTCT1162150.032.55206349
Produced by FastQC (version 0.11.5)