FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160056105
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG2112540.13198746776950496No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGGGGT1919990.11995731121908783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA925000.041.0876349
GCCGTAT949550.040.00369347
TCGCCGT979600.038.77556245
GATCGGA1005900.038.309831
GTCGCCG998050.038.20129844
CCGTATC1018050.037.34522648
TTGAACG717700.036.726847
TGGTCGC1055100.036.16224342
AGAGCGT1075700.035.692668
GCGTCGT1117200.034.12819311
CGCCGTA1125650.033.88504446
GTATCAT1129350.033.8737750
AACGCTG776450.033.63423510
GTAGATC1166050.033.33701731
TCGTGTA1140000.033.2697214
TCGGTGG1182650.032.83637638
GTCGTGT1160000.032.7794513
AGATCGG539200.032.7207871
GGTCGCC1173700.032.54924843
AAGAGCG1205350.032.1320157
Produced by FastQC (version 0.11.5)